From: Department of Health and Human Services, State of Alaska

SARS-CoV-2 Virus Real-Time PCR

The Alaska State Public Health Laboratories in Anchorage and Fairbanks use real-time reverse-transcriptase polymerase chain reaction (rtRT-PCR) to accurately identify the presence or absence of SARS-CoV-2 viral genetic material (ribonucleic acid, or RNA) in clinical specimens collected from the respiratory tract of patients.

At the Alaska State Public Health Laboratories, our main PCR assay for SARS-CoV-2 has an end point at 37 cycles, and our backup assay to maintain capacity has an end point of 40 cycles.

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